[Histonet] tissue artifact

Tony Reilly Tony_Reilly <@t> health.qld.gov.au
Mon May 2 19:39:09 CDT 2011

Hi Cindy
This artifact can also be as a result of the local anesthetic.  In this case there is nothing you can do to prevent it.  I am not sure why it does not appear in all samples.

Tony Reilly  B.App.Sc. , M.Sc.
Chief Scientist, Anatomical Pathology
Pathology Queensland-PA Laboratory
Clinical and Statewide Services Division| QueenslandHealth
Level 1, Building 15,Princess Alexandra Hospital
Ipswich Road,WOOLLOONGABBA  Qld4102
Ph: 07 3176 2412
Mob: 0402 139411
Fax: 07 3176 2930
Email: tony_reilly <@t> health.qld.gov.au
Web:  www.health.qld.gov.au/qhcss/

>>> cindy dewar <cindy38017 <@t> yahoo.com> 4/27/2011 5:54 am >>>
I work in a dermpath lab. One day last week we had a few slides that had "vacuoles" or holes next to the nucleus. We had over 600 hundred cases, and it only happened to a few of them. There seems to be nothing in common with them. The biopsies came from different Dr's offices, different companies manufactured the formalin bottles, etc. It only happened one day and since then there have been no issues. Since it does not involve all of the specimens, it doesn't seem to be processing or staining. Could it be from sectioning due to freeze spray? too many sponges?. If anyone has any ideas as to what could cause this, I would greatly appreciate it.
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