[Histonet] Re: Weigert's Iron Hematoxylin

Johnson, Nacaela Nacaela.Johnson <@t> USONCOLOGY.COM
Wed Mar 30 15:03:50 CDT 2011

Iron hematoxylin is more resistant to decolorization than aluminum
hematoxylin.  Since there are so many acid steps that follow the
hematoxylin, it is wise to use the Weigert's so you do not have to worry
about decolorizing the other tissue elements too far.  However, if you
optimize your stain to the use of the Gill's and the pathologists agree
that staining intensity is optimal then it should be fine.  I wouldn't
do it without optimizing first.  So overall, the two are not exactly
interchangeable since some of the subsequent staining steps may have to
be altered. 

Nacaela Johnson, HTL (ASCP)
KCCC Pathology
12000 110th St., Ste. 400
Overland Park, KS 66210
Office:  913-234-0576
Fax:  913-433-7639
Email:  Nacaela.Johnson <@t> USOncology.com

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Blundon,
Sent: Wednesday, March 30, 2011 2:55 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: Weigert's Iron Hematoxylin

Hi Histonetters,


I have a quick question regarding Weigert's Iron Hematoxylin and Gill's
3 Hematoxylin. One of the guys here thinks that we can interchange these
2 stains. I am attempting to perform a Masson Trichrome stain and it
calls for Weigert's Iron Hematoxylin. This person in my lab says to just
use Gill's Hematoxylin in place of the Weigert's stain. Is this right?


Thanks for your help



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