[Histonet] Vaginal Smears-Fixation

Amber Tyler atyler <@t> pcg.wustl.edu
Fri Mar 25 11:06:00 CDT 2011

Good Morning,

I am doing a study on changes in estrous cycling in mice over the long term.
I will be collecting vaginal smears daily and staining with new methylene
blue. I need to be able to keep the slides "fresh" for viewing for
approximately a week.

Does anyone have any advice on the best manner of fixing/mounting procedures
for this?
I have little experience with this. In previous studies, the slides were
only needed briefly and discarded.

Thank you,
Amber N. Tyler, Ph.D.
Staff Scientist
Anatomy & Neurobiology

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