[Histonet] GREAT B5 substitute

Akemi Allison akemiat3377 <@t> yahoo.com
Thu Mar 24 15:03:40 CDT 2011

Having worked and developed products in the Biotech arena, not all  
products are disclosed.  If they are, you lose the competitive edge.   
Here is the link to the the MSDS and information BCC discloses;


Section 2.  Composition and Information on Ingredients
Name CAS# % by Weight Exposure Limits
1) Water
2) Formaldehyde 37%
3) Zinc Salts
4) Selected Buffers
Not Available
Not Available

 >0.5% - <1%
Not Available
Not Available
OSHA PEL 0.75ppm TWA
Not Available
Not Available

Akemi Allison BS, HT (ASCP) HTL
Phoenix Lab Consulting
Tele: 408.335.9994
E-Mail: akemiat3377 <@t> yahoo.com

On Mar 24, 2011, at 12:28 PM, Johnson, Nacaela wrote:

> Are the contents of the B Plus Fixative proprietary?  Is it an AZF  
> type
> of fixative?  Did you try any ISH on the test runs?
> Thanks,
> Nacaela Johnson, HTL (ASCP)
> Histotechnologist
> KCCC Pathology
> 12000 110th St., Ste. 400
> Overland Park, KS 66210
> Office:  913-234-0576
> Fax:  913-433-7639
> Email:  Nacaela.Johnson <@t> USOncology.com
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Akemi
> Allison
> Sent: Thursday, March 24, 2011 12:08 PM
> To: histonet
> Subject: [Histonet] GREAT B5 substitute
> Hi All,
> I used to use B5, Zenker's and Helly's for years on LN and BM  
> biopsies.
> I wanted to stop using mercury in our department, but didn't want to
> compromise the quality of the results we were getting.
> I was interested in using fixatives and decalcification products that
> would not effect IHC results. At the suggestion of Dr. Elaine Jaffe,
> Chief Hematopathologist from NIH, I tried 2 products from BBC
> Biochemical.  I did parallel studies on bilateral BM bx's using B*Plus
> Fixative(tm) and RapidCal*Immuno(tm), and then B5 fixative and EDTA
> decal solution, and then Zenker's fixative.  We then did parallel
> studies for IHC.  We ran a panel of Ab's on specimens which were  
> treated
> with each of the solutions.
> The pathologists concluded that B*Plus Fixative(tm) produces superb
> fixation for hematopoietic tissues and the RapidCal*Immuno(tm) does  
> not
> interfere with IHC staining.  In some cases, the results on the IHC's
> were better on some of the Ab's using these products too.
> It only takes 2-3 hours of fixation time for BM biopsies.  After
> prefixing, they can go directly into RapidCal*Immuno(tm) for 45  
> minutes
> to 1 hour, washed in running water for 5 minutes, and then continue to
> be processed as normal starting off in 10% formalin.  Just a note:  
> This
> product is a rapid decalcifier designed to preserve immunogenicity  
> at a
> maximum while accomplishing rapid decalcification. It is designed
> specifically for bone marrow biopsies and LN biopsies.
> By the way, I am not on BBC's payroll.  I am always on a quest to
> improve patient care!
>  Akemi Allison BS, HT(ASCP)HTL
> Director
> Phoenix Lab Consulting
> E-Mail: akemiat3377 <@t> yahoo.com
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