[Histonet] RE: PAS for basement membrane

[Bartlett, Jeanine (CDC/OID/NCEZID)]

We wash after Schiff's for 10 minutes in regular temp tap water.  Don't know if that helps or not......

Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590 
jeanine.bartlett <@t> cdc.hhs.gov

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Martin, Erin
Sent: Tuesday, March 15, 2011 10:17 AM
To: histonet
Subject: [Histonet] PAS for basement membrane

Hi all,

I'm having a problem with my PAS stain.  My pathologist says that glycogen and fungus stain beautifully but basement membranes are pale.  We are using 0.5% periodic acid for 10 minutes and schiff's for 25 minutes followed by 5 min rinse in warm/hot water.  Any thoughts?  Thank you in advance!

Thanks
Erin

Erin Martin, Histology Supervisor
UCSF Department of Dermatopathology
415-353-7248

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet