[Histonet] VALIDATION of a new clone

Mark Tarango marktarango <@t> gmail.com
Mon Mar 14 13:21:50 CDT 2011

Hi Jim,

If it's a new clone then a new valiation should be performed.  I know of
many instances where one clone stains something that another doesn't.

If this is regarding Ventana not selling certains clones, those clones are
still available from Cell Marque directly.


On Mon, Mar 14, 2011 at 11:16 AM, Vickroy, Jim <Vickroy.Jim <@t> mhsil.com>wrote:

> I am wondering what others  are doing when they validate a new clone of a
> antibody that you have used in the lab for quite sometime.  When we
> introduce a new antibody obviously we go through a pretty extensive
> validation process including sampling of around 20 - 30 specimens, some
> presumed positive cases and some presumed negative cases.   We also try to
> include in the positive cases some that are strongly positive and others
> that are weakly positive.
> Lately several of our regular antibodies are now no longer available in the
> same clone.  I am interested in how different labs would validate the new
> clone to be used in the lab.  It would seem to me that the validation
> process would not have to be as extensive since we have already established
> that the antibody works well in our laboratory.   I know if it was the same
> clone but a different lot we would just do a comparison of the old lot with
> the new lot, but what about a different clone?   I realize that the methods
> are going to be diverse but I would appreciate hearing from others.
> James Vickroy BS, HT(ASCP)
> Surgical  and Autopsy Pathology Technical Supervisor
> Memorial Medical Center
> 217-788-4046
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