[Histonet] RE: Preferred green counterstain for GMS?

Reilly, Laurie laurie.reilly <@t> jcu.edu.au
Tue Mar 8 16:21:39 CST 2011

My mentors way back taught us to use Methyl Green as a counterstain for GMS because it also stains nuclei, but it has to be cleared with chloroform to remove Methyl Violet contamination.

Regards,   Laurie.

Mr. Laurie REILLY
School of  Veterinary and Biomedical Sciences
James Cook University
Townsville  Qld.  4811

Phone 07 4781 4468

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Langsdorf, Aliete E.
Sent: Wednesday, 9 March 2011 2:38 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Preferred green counterstain for GMS?


What is your preferred green counterstain for GMS?
In the kit I have, it suggests Fast Green FCF, Light Green SF Yellowish or
Which should I buy?

Any help is appreciated!

Senior Research Technician
Medical Oncology
Dana-Farber Cancer Institute

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