[Histonet] Mouse cardiac myosin antibodies

Mark Elliott Mark.Elliott <@t> hli.ubc.ca
Wed Mar 2 16:59:17 CST 2011

I have been asked to stain mouse hearts for cardiac myosin. I have tried the antibody from Abcam (ab50967-Mouse monoclonal to heavy chain cardiac myosin) with no luck. I am using the Mouse on Mouse kit from Biocare and it worked well for me for some other mouse antibodies on mouse tissue but doesn't want to work on this antibody.  I have tried different retrieval solutions (pH6 and pH9, as well as Biocare rodent Decloaker). Anyone use this antibody?  Any suggestions for another antibody greatly appreciated.

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