[Histonet] Fat tissue

[sgoebel <@t> mirnarx.com]

For the most part you cannot section fat on a cryostat because of the
oilyness (is that a word).  You can fix it and section it?
Good Luck

Sarah Goebel, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Margaret
Blount
Sent: Wednesday, March 02, 2011 4:54 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Fat tissue

Has anyone experience of cryostat sectioning of fat tissue? This would
be rodent fat pads, particularly the gonadal fat pad which usually
consists largely of adipocytes.

 

What temperature would you cut at? Is there anything that could be used
to infiltrate the tissue prior to sectioning to provide more support?

 

Regards

 

Margaret

 

Miss Margaret Blount

Histology Manager

Metabolic Research Laboratories

Level 4 Institute of Metabolic Science

Box 289, Addenbrooke's Hospital

Hills Road, Cambridge, CB2 0QQ

 

Tel 01223 769061/336079

 

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