[Histonet] Cutiing of dextran beads (snap frozen)

Barone, Carol cbarone <@t> NEMOURS.ORG
Thu Jun 30 11:36:52 CDT 2011


Histonetters....We have received some dextran beads (coated with cells)
that are "snap frozen"...for cutting.

We have done these beads before in histogel/paraffin...but are curious
as to how best to handle them snap frozen. We have a nice bead with no
cells on our first to snap frozen sample blocks....Help? Is it the
temperature? We cut at -25 on average.


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