[Histonet] Macrophage marker in sheep for IHC

[Vivek Saroha]

Hi there,
I am very new to basic sciences research and am planning an experiment to quantify and compare adipose tissue macrophage infiltration in sheep model using immunohistochemistry. I am specifically interested in CD11c as a marker of M1 activation of Macrophages. I have been advised that it is not easy and previous effort by my predecessors have not been very successful in this!
>From the general google, histonet/histosearch and pubmed search. I have made a list of potential candidates:

1.    Clones PG M1 and Clone KP1
(Source : Histonet : http://www.histosearch.com/histonet/Nov03A/RE.HistonetSeepmacrophage.html)


2.    EMB11, a mouse anti-human CD68 mAb (Dako, Carpenteria, CA)
(source: published paper: http://www.ncbi.nlm.nih.gov/pubmed/16460804 )


 1.  Another CD68 antibody
Source: http://www.ingentaconnect.com/content/ocean/ajra/2011/00000025/00000002/art00024)

 1.  Iba1 (ionized calcium binding adaptor molecule 1) is a 17-kDa EF hand protein that is specifically expressed in macrophages/microglia and is upregulated during the activation of these cells.[Wikipedia]
(source: I do not remember where I found this one from but Abcam has several antibodies none of which are predicted for sheep)
I am writing to ask for suggestions about which one to go for and any other tips about searching for antibodies or performing IHC in paraffin fixed adipose tissue/liver.
Any ideas will be gratefully appreciated as I am very new and isolated in this aspect.
Best wishes
Vivek

Dr Vivek Saroha
Clinical lecturer in Child health
University of Nottingham
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