[Histonet] Retirement
O'Donnell, Bill
billodonnell <@t> catholichealth.net
Mon Jun 20 09:31:01 CDT 2011
You had COTTON in your pipettes? We used hollowed out reeds with a bit
of papyrus in one end. No calibration.... But plenty accurate enough for
histo in those days.
However, there are a few things I do not miss from back in da' day.
Hand-stropping a knife for an hour, only to have it nicked by a staple
in the next hour.
-Bill
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
histotech <@t> imagesbyhopper.com
Sent: Sunday, June 19, 2011 7:48 PM
To: Paula Sicurello
Cc: histonet <@t> lists.utsouthwestern.edu; Amos Brooks
Subject: Re: [Histonet] Retirement
And remember mouth pipeting? "oops, that's the cotton..."
Eating and smoking in the lab was the norm.
Our alcohol had the tax stamp on it! :o)
Michelle
On Jun 19, 2011, at 4:19 PM, Paula Sicurello <patpxs <@t> gmail.com> wrote:
> Sheesh! We used to have people smoke while working with propylene
oxide.
>
> Eating in your control pigs was part of the benefit of being a
> graduate student to save on grocery money.
>
> Film? My TEM used glass plates.
>
> Lab mates used to routinely drink diet coke and 100% ethanol on
Fridays.
>
> Wearing closed toed shoes was for wimps, you were just fast if you
> dropped a steel wedge blade.
>
> We even wrote using the entire word and proper grammar, none of this
> acronym stuff for us.
>
> Retirement? What's that?
>
> Paula :-)
>
> On Sun, Jun 19, 2011 at 12:31 PM, Amos Brooks <amosbrooks <@t> gmail.com>
wrote:
>> Agarose Gels!
>> ... Listen you whipersnapper Agarose is the easy way out. When I
>> learned it we used to have to make up our own polyacrylamide gels.
>> That was after having to walk to work up hill both ways in 30 feet of
snow!
>>
>> (No nearer retirement)
>> Crotchety Amos
>>
>>
>>
>> Message: 7
>> Date: Fri, 17 Jun 2011 13:24:12 -0400
>> From: Emily Sours <talulahgosh <@t> gmail.com>
>> Subject: Re: [Histonet] Retirement
>> To: histonet <@t> lists.utsouthwestern.edu
>> Message-ID: <BANLkTineJxtXYoP-byfweuxn3yw-ff3Qsg <@t> mail.gmail.com>
>> Content-Type: text/plain; charset=UTF-8
>>
>> Retirement? I think by the time I get to that point, social security
>> will have run out.
>> Then again, technology will be so advanced, I can tell stories about
>> the old days, where I logged on to the bbs by modem to post messages
>> to my friends and typed in my own html coding.
>> We didn't have google when I was young!! Our cameras used film! And
>> you couldn't see how bad your pictures were until you developed that
film!!
>> There was no PCR to sequence your DNA, you ran an agarose gel and
>> hoped for the best!! You could drink the 100% ethanol, there was no
>> denaturing! (okay that was before my time) You could smoke in the lab
>> while you sectioned without gloves!! (okay that was too)
>>
>> Emily
>>
>> A great book should leave you with many experiences, and slightly
exhausted.
>> You should live several lives while reading it.
>> -William Styron
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>
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