[Histonet] staining of tissue culture plates

[Amos Brooks]

Hi,
   This is actually fairly easy. It is already an aqueous environment. Pour
off the buffer and add hematoxylin (time is dependent upon the type of
hematoxylin used, basically the same as staining slides) pour off the
hematoxylin and rinse a couple times with water add acid alcohol briefly
while swirling the plate (10 seconds should suffice) rinse with water add 1%
ammonium hydroxide 30, seconds should be plenty. Rinse in water then 95%
ETOH. Add eosin, 30 seconds should suffice. Rinse in 95% then 100% ETOH. Let
it air dry and coverslip with resinous mounting media if you want to, Some
PIs like to just read directly from the plate. Personally I think the
coverslip looks better, but I would be making it for them not myself. (An
important difference)

Good Luck,
Amos


Message: 5
Date: Fri, 17 Jun 2011 12:12:10 -0500
From: Patricia F Lott <plott <@t> uab.edu>
Subject: [Histonet] staining of tissue culture plates
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Dear Gayle:

I have a PI who wants us to do H&E staining on tissue culture plates.  Have
you ever done this?  Can you send me a protocol?

Thanks,
Patty Lott