[Histonet] Pre-made paraformaldehyde question

[Geoff McAuliffe]

Suh et al.

I remember that the dogma on paraformaldehyde was that it must be made 
fresh. However, I also remember that someone has actually compared fresh 
to "old" (a week or two) and found no difference. This was published in 
a peer-reviewed journal, I just don't remember which one.

Geoff

On 6/8/2011 2:14 PM, Sherwood, Margaret wrote:
> I don't believe that anyone responded to your inquiry.  I used to make up a
> Karnovsky's fixative (for TEM) (paraformaldehyde/glutaraldehyde) using the
> powder form of paraformaldehyde.  It is highly toxic.  No one that I know of
> does this anymore (or shouldn't).  Now I buy the 16% solution of
> paraformaldehyde (comes in sealed 10ml ampules).  I also buy my glutaraldehyde
> solution that way.  Then I make whatever dilution I need.  Much safer and
> simpler this way.
>
> Peggy
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Suhyoung Jeong
> Sent: Monday, June 06, 2011 8:39 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Pre-made paraformaldehyde question
>
> Dear Histo people,
>
> In my old lab, I have been taught that 4% PFA has to be always made fresh
> (maybe a day before max). Here in my new lab, I find that people use 32%
> pre-made PFA from EMS (without expiry date) and only dilute it on the day of
> use.
>
> Does anyone have experience (or thoughts) with this issue?
>
> Thank you in advance
>
> All the best, Suh
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> The information in this e-mail is intended only for the person to whom it is
> addressed. If you believe this e-mail was sent to you in error and the e-mail
> contains patient information, please contact the Partners Compliance HelpLine at
> http://www.partners.org/complianceline . If the e-mail was sent to you in error
> but does not contain patient information, please contact the sender and properly
> dispose of the e-mail.
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>


-- 
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583
mcauliff <@t> umdnj.edu
**********************************************