[Histonet] Pre-made paraformaldehyde question

[Sherwood, Margaret]

I don't believe that anyone responded to your inquiry.  I used to make up a
Karnovsky's fixative (for TEM) (paraformaldehyde/glutaraldehyde) using the
powder form of paraformaldehyde.  It is highly toxic.  No one that I know of
does this anymore (or shouldn't).  Now I buy the 16% solution of
paraformaldehyde (comes in sealed 10ml ampules).  I also buy my glutaraldehyde
solution that way.  Then I make whatever dilution I need.  Much safer and
simpler this way.

Peggy 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Suhyoung Jeong
Sent: Monday, June 06, 2011 8:39 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Pre-made paraformaldehyde question

Dear Histo people,

In my old lab, I have been taught that 4% PFA has to be always made fresh
(maybe a day before max). Here in my new lab, I find that people use 32%
pre-made PFA from EMS (without expiry date) and only dilute it on the day of
use.

Does anyone have experience (or thoughts) with this issue?

Thank you in advance

All the best, Suh
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