[Histonet] Pie crust effect on frozen Muscle H&E

Mon Jun 6 13:35:00 CDT 2011

Has anyone ever heard of “pie crust” effect on frozen muscle slides? My pathologist has found the picture in a book calling our artifact “pie crust” but it gave no explanation as to the cause or fix. Help!

Jennifer Harvey, HT(ASCP) QIHC
Vanderbilt University Medical Center
Neuropathology Lab Supervisor
C-2309 Medical Center North
Nashville, TN  37232-2561
Phone: 615-343-0083
Fax: 615-343-7089