[Histonet] why no replay????

mohamed abd el razik k84as <@t> yahoo.com
Sat Jun 4 11:00:10 CDT 2011


thanx to all kind replays from sgoebel, Paula Pierce and JR R. 
but i know that Alcian blue 2.5 stain most acidic mucins and pH 1 stain sulfated one. my quistion is:  i found in several serial section on intestine that 100 goblet cell react to AB ph 1 and only 50 cell reacts to AB 2.5 why?????? it should be the reverse numbers as at pH 2.5 all acidic sulfated or non sulfated are stained. in other words, ph 2.5 should stain 100 cell as ph 1 stains 100 cell at least. so i'm confused about that and replay or staining several times according to Bancroft and Farida and these was confirmed


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