[Histonet] Slide Bright Question

Christopher Conlisk cconlisk <@t> kcskincenter.com
Fri Jun 3 13:17:37 CDT 2011


Hello Everyone,
    I have worked in labs that use Xylene my entire career and I just
started at a Lab That only uses a Xylene Substitute "Slide-Bright". I am
having problems with the H&E. After staining and coverslipping (The slides
look fine innitially), Then about 5-10 minutes after coverslipping the Eosin
starts bleeding out all around the tissue. I have asked several of my
Histotech Friends that are old timers and they say that Xylene Substitutes
are awful at deparrifinization and awful at clearing. They told me that the
alcohol isnt getting thoroughly cleared in the "Slide Brite" and then it is
eventually leeching out after coverslipping??? Is this true and does anyone
have any guidance for this issue? We also run MOHS slides on the same
stainer and I keep all the reagents clean as a whistle. I really hate Xylene
Substitute's....

Thanks

C.S. Conlisk HT(ASCP), PBT(ASCP)
Kansas City Skin and Cancer Center


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