[Histonet] Antibody suggestions for dog and cat

[Goins, Tresa]

Hi Mark -

We have had good results with: 
	Vimentin Clone V9 (CM048), Factor VIII (PAb-CP039) and pan-Cytokeratin, Clone Lu-5 (CM043) from Biocare
	CD3 C7930 from Sigma
	CD79a Clone HM47/A9 (MS357), Melan A (MS799) from Thermo Scientific - no luck with CD20.
	CD117 (RB9038) from Thermo Scientific - data sheet states heat retrieval is required, but we get good results without retrieval.

I have also found that while Melan A is excellent in canine tissue, S100 (PA1-38584, ThermoSci) works better in feline tissue - more positive hits with higher intensity of specific staining. 

Best regards,


Tresa Goins
Veterinary Diagnostic Lab
South 19th and Lincoln
Bozeman, MT 59718
406-994-6353 - phone
406-994-6344 - fax

 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Mark Tarango
Sent: Monday, July 25, 2011 12:34 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Antibody suggestions for dog and cat

Could anyone suggest suituble antibodies for the following markers in dog
and cat tissue:

Vimentin, Pankeratin, CD3 and CD20, CD18, MelanA, Factor VIII

Also could use a suggestion for C-kit in Dog tissue only.

thanks

Mark
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