[Histonet] Controls with patient specimen on same slide

Setlak, Lisa LSetlak <@t> childrensmemorial.org
Tue Jul 19 14:56:56 CDT 2011


We often do the same or use separate slides. We used to use the slides with the designated "control" area but had some problems with the reagents getting to all areas of the slide.
Lisa

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Mark Tarango
Sent: Tuesday, July 19, 2011 2:43 PM
To: Rathborne, Toni
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Controls with patient specimen on same slide

Hi Toni,

We put the control on the same slide and after it's stained and coverslipped
we draw a line to seperate the control and patient tissue with each side of
the line having either a "C" or "P" written on it.

Mark
On Tue, Jul 19, 2011 at 12:27 PM, Rathborne, Toni <
trathborne <@t> somerset-healthcare.com> wrote:

>
> Hi,
> I'm interested in knowing how many of you are performing ihc with the
> control tissue and the patient tissue on the same slide. I have seen slides
> available which have designated areas for each tissue to be placed so there
> will not be any confusion. If you're doing it, have you encountered any
> problems? What benefits have you noticed since implementing this process?
> Are your pathologists in favor of this?  If you're not, why not?
> Thanks,
> Toni
>
>
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