[Histonet] RNAlater buffer & fixation/embedding in OCT

[Megan French]

Has anyone successful fixed & embedded tissues in OCT after using
RNAlater buffer? I've found a paper that says this doesn't affect tissue
integrity but I need for info regarding protocol (To defrost? Fixative?
Timing? Temperature Etc) ...and more than anything feedback from someone
who has used/uses this product.

 

Product: http://www.ambion.com/techlib/resources/RNAlater/

Paper: http://www.nature.com/modpathol/journal/v14/n2/pdf/3880267a.pdf

 

 

Miss Megan French BBiomedSc(Deakin); BSc(Hons)(Melbourne)

Research Assistant

Surgical Research; Infection & Immunity Theme

 

Murdoch Children's Research Institute

The Royal Children's Hospital
Flemington Road Parkville Victoria 3052 Australia
E megan.french <@t> mcri.edu.au

 

 

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