[Histonet] Light microscopy to get orientation for fluorescent

[Adam .]

Try differential interference microscopy (DIC). Many fluorescent scopes will
have this as an option. It will give you faint outlines of the cells.

Adam

On Sun, Jul 17, 2011 at 1:01 AM, Otto Strauss <olstrauss <@t> gmail.com> wrote:

> I am trying to do fluorescent immunohistochemistry on liver tissue.
> Unfortunately because the liver is such a homogenous tissue it is difficult
> to get an appropriate orientation with just fluoroscopic views.
>
> I was wondering if there is a way of staying with non fluorescent dyes(like
> H&E) so that I can get a picture with light microscopy, to have orientation
> within the liver when viewing it with fluorescent microscopy?
>
> Regards.
>
> Otto
> Auckland
> NZ
>
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