[Histonet] Light microscopy to get orientation for fluorescent

[Otto Strauss]

I am trying to do fluorescent immunohistochemistry on liver tissue. Unfortunately because the liver is such a homogenous tissue it is difficult to get an appropriate orientation with just fluoroscopic views. 

I was wondering if there is a way of staying with non fluorescent dyes(like H&E) so that I can get a picture with light microscopy, to have orientation within the liver when viewing it with fluorescent microscopy? 

Regards. 

Otto 
Auckland 
NZ