[Histonet] Antigen retrieval survey

[Leiker, Merced]

96 degree water bath for half an hour in either citrate buffer (pH 6) or Tris-HCl (pH 9), depending on which gives better retrieval for that antibody.




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Andrea T. Hooper
Sent: Wednesday, July 13, 2011 11:10 PM
To: Histonet
Subject: [Histonet] Antigen retrieval survey


Hi All,
 
I am doing a survey and will be happy to compile results and share if folks will respond! What is your favorite antigen retrieval method and/or panel?
 


Buffer

Source/composition

Temperature

Device
 
Thanks, Andrea
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