[Histonet] Antigen retrieval survey

[Goins, Tresa]

I hate to be pessimistic, but different clones of monoclonal antibodies can have different optimum retrieval requirements, not to mention tissue type, source species, etc.  

What I do believe however, is that enzymatic retrieval is under-sold - no equipment purchase is necessary so you will not see companies advocating their use.  Nearly half of the antibodies we use are optimally retrieved with enzymes (pronase, trypsin, pepsin) rather than heat retrieval.  


Tresa Goins
Veterinary Diagnostic Lab
South 19th and Lincoln
Bozeman, MT 59718
406-994-6353 - phone
406-994-6344 - fax

Tresa

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Andrea T. Hooper
Sent: Wednesday, July 13, 2011 9:10 PM
To: Histonet
Subject: [Histonet] Antigen retrieval survey


Hi All,
 
I am doing a survey and will be happy to compile results and share if folks will respond! What is your favorite antigen retrieval method and/or panel?
 


Buffer

Source/composition

Temperature

Device
 
Thanks, Andrea
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