[Histonet] Re: Embedding Media

[histotech <@t> imagesbyhopper.com]

Amita,

The paraffin that we use is also a Leica product. It was formerly sold under the Surgipath label, but since to companies have merged together, it now sells under the Leica name. It is a "beaded" pellet paraffin and has a version suitable for infiltration and a version suitable for embedding.  The box of the embedding paraffin suggests it can be utilized for *both* purposes, however we do not choose to use it that way.  So yes, we use two different types of paraffin.

Hope that helps!
Michelle

On Jul 11, 2011, at 2:07 AM, amitapandey <@t> torrentpharma.com wrote:

> I continuation of same discussion , i would like to have clarification for 
> our lab..we use ....beaded paraffin "Leica Paraplast"  both for processing 
> and embedding . Do you advice us to use different paraffin for both steps?
> 
> 
> Paula, Please share if you get any feed back on Richard Allan product.
> 
> Amita
> 
> 
> From:   "Tim Higgins" <thiggins <@t> cddmedical.com>
> To:     <histonet <@t> lists.utsouthwestern.edu>
> Date:   07/08/2011 11:34 PM
> Subject:        [Histonet] Re: Embedding Media
> Sent by:        histonet-bounces <@t> lists.utsouthwestern.edu
> 
> 
> 
> Hi Paula,
> 
> Obviously there are no "compression free" paraffin's on the market.  Look
> for a paraffin with a higher polymer content, this makes the paraffin 
> firmer
> and less likely to compress.  Other factor for into section compression 
> but
> paraffin is a good place to start.
> 
> The higher polymer paraffin's are not as suited for processing, it is more
> for the embedding portion of the process.
> 
> Use a paraffin with lower polymers content in the processor and higher for
> embedding and you will like the results.
> 
> 
> Tim
> 
> 
> Date: Fri, 8 Jul 2011 09:44:51 -0700
> From: "Paula Lucas" <plucas <@t> biopath.org>
> Subject: [Histonet] Embedding Media
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <9251EDA88CA748D6861A0C3BB0E32FCB <@t> biopath.local>
> Content-Type: text/plain; charset="us-ascii"
> 
> Hello,
> 
> 
> 
> We are considering a switch to a different brand of paraffin and this is
> because I feel we are having too many compressions in some of our tissue
> sections.  Currently, we use Tissue Path Paraplast, regular.
> 
> 
> 
> I would like to get feedback from you as to what you prefer.  Looking on
> line, Richard Allan has a product called Signature Series Paraffin that
> comes in a type L that offers compression-free sections and I was also
> hoping to get any feedback on that product.
> 
> 
> 
> I would greatly appreciate any suggestions and thoughts.
> 
> Thanks in advance,
> 
> Paula
> 
> 
> 
> 
> 
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