[Histonet] Re: Embedding Media

[amitapandey <@t> torrentpharma.com]

I continuation of same discussion , i would like to have clarification for 
our lab..we use ....beaded paraffin "Leica Paraplast"  both for processing 
and embedding . Do you advice us to use different paraffin for both steps?


Paula, Please share if you get any feed back on Richard Allan product.

Amita


From:   "Tim Higgins" <thiggins <@t> cddmedical.com>
To:     <histonet <@t> lists.utsouthwestern.edu>
Date:   07/08/2011 11:34 PM
Subject:        [Histonet] Re: Embedding Media
Sent by:        histonet-bounces <@t> lists.utsouthwestern.edu



Hi Paula,

Obviously there are no "compression free" paraffin's on the market.  Look
for a paraffin with a higher polymer content, this makes the paraffin 
firmer
and less likely to compress.  Other factor for into section compression 
but
paraffin is a good place to start.

The higher polymer paraffin's are not as suited for processing, it is more
for the embedding portion of the process.

Use a paraffin with lower polymers content in the processor and higher for
embedding and you will like the results.


Tim


Date: Fri, 8 Jul 2011 09:44:51 -0700
From: "Paula Lucas" <plucas <@t> biopath.org>
Subject: [Histonet] Embedding Media
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <9251EDA88CA748D6861A0C3BB0E32FCB <@t> biopath.local>
Content-Type: text/plain; charset="us-ascii"

Hello,



We are considering a switch to a different brand of paraffin and this is
because I feel we are having too many compressions in some of our tissue
sections.  Currently, we use Tissue Path Paraplast, regular.



I would like to get feedback from you as to what you prefer.  Looking on
line, Richard Allan has a product called Signature Series Paraffin that
comes in a type L that offers compression-free sections and I was also
hoping to get any feedback on that product.



I would greatly appreciate any suggestions and thoughts.

Thanks in advance,

Paula





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