[Histonet] Isopropyl alcohol

[histotech <@t> imagesbyhopper.com]

Rene,
I was just as surprised!  When we purchased the mw, we were led to believe that we could use the recycled alcohol, but I was told later that we should not for quality reasons - that recycled is never 100% pure and that water contamination will adversely affect the processing.

So, can I use it on my H&E stainer without negatively affecting the stain quality?

As always, thanks for you thoughts,
Michelle


On Jul 5, 2011, at 11:58 AM, Rene J Buesa <rjbuesa <@t> yahoo.com> wrote:

> First of all, if you recycle correctly your 2-propanol there is no objective reason why you cannot use it in your MW processor, unless your provider wants you to buy your 2-propanol from them.
> René J.
> 
> --- On Tue, 7/5/11, histotech <@t> imagesbyhopper.com <histotech <@t> imagesbyhopper.com> wrote:
> 
> From: histotech <@t> imagesbyhopper.com <histotech <@t> imagesbyhopper.com>
> Subject: [Histonet] Isopropyl alcohol
> To: "Histonet <@t> Lists. Utsouthwestern. Edu" <histonet <@t> lists.utsouthwestern.edu>
> Date: Tuesday, July 5, 2011, 10:42 AM
> 
> We recycle our isopropyl alcohol. We have been told by our microwave manufacturer that we cannot use the recycled alcohol on the microwave processor.
> 
> As a result, I am trying to find additional uses for the recycled isopropyl.  Is there any reason why I can't use the iso in place of ethanol when running the slides down to water on the H&E stainer? 
> 
> Any others uses?
> 
> Any input is greatly appreciated!  :o)
> 
> Michelle
> 
> 
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