[Histonet] Dog Cartilage Safranin-O Stain

[Michelle Aono]

I am staining the joints of multiple species with Safranin-O/Fast
Green/Hematoxylin and consistently see a much weaker stain with the
SO/FG in dog cartilage.  At first I thought it had to do with the
thickness of the sections, but even as 6u it's still very light.  I'm
staining it at the same time as the other species so it's not variations
in staining time, solution age, etc...

Has anyone else seen this effect?  
Any ideas on how to fix this and why this is?  
Species proteoglycan compositional differences?

Thanks!

Histology Newbie,
:) Shelly

~~~~~~~~~~~~~~~
Michelle (Shelly) Aono
Histology Technician IV
CVM & AU Staff 
Advisory Council Representative
Auburn University
CVM-APP
212 Greene Hall, Auburn, AL 36832
(334) 844-5594