[Histonet] Bacterial contamination

[histotech <@t> imagesbyhopper.com]

Another place to check is the "holding" water that the slides rest in just prior to staining.  We run our slides to water on the H&E stainer and then transfer the rack to a "holding" water dish.  We bleach this dish nightly, as we have found it contaminated in the past.  We don't know what caused the contamination, but we have not it since the bleaching started!  ;o)

Good luck!

Michelle


On Jan 27, 2011, at 1:41 PM, BSullivan <@t> shorememorial.org wrote:

> One place you need to look is the floatation bath where you cut your
> slides.
> 
> Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.)
> AP Supervisor
> Shore Memorial Hospital
> 609-653-3590
> 
> 
> Speak only well of people and you need never whisper
> 
> 
> 
>             "Breeden, Sara"                                               
>             <sbreeden <@t> nmda.nm                                             
>             su.edu>                                                    To 
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>             lists.utsouthwest                                          cc 
>             ern.edu                   "Ragsdale, John"                    
>                                       <JRagsdale <@t> nmda.nmsu.edu>           
>                                                                   Subject 
>             01/27/2011 01:39          [Histonet] Bacterial contamination  
>             PM                                                            
> 
> 
> 
> 
> 
> 
> 
> 
> 
> My pathologist tells me I have floating bacteria in both special stains
> I did this morning (GMS and Gram); some slides have these floating
> critters and some don't.   Because the only common solutions are those
> for processing and deparaffinization and because these bacteria appear
> to be floating above the plane of the tissue - I can't figure out where
> to start looking.   My DI water is from a central source and is
> routinely quality-checked, and this is a new building (Sep. 2010)  I
> don't want to blame that. Knowing full well that I am probably
> overlooking the obvious, I'm asking for help figuring this out.  I need
> a Sputnik Moment.  Thanks!
> 
> 
> 
> Sally Breeden, HT(ASCP)
> 
> New Mexico Department of Agriculture
> 
> Veterinary Diagnostic Services
> 
> 1101 Camino de Salud NE
> 
> Albuquerque, NM  87102
> 
> 505-383-9278 (Histology Lab)
> 
> 
> 
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