[Histonet] Oil Red O for FS on Muscle

[Catherine Simonson]

Hello Akemi,

One thing though, how do you "mix" your oil red o?  just a stir bar?  I
found that if you don't shake it very vigorously for about 30 seconds before
filtering you will get that background staining.  Remember to shake it
(shaken, not stirred)

Catherine

On Thu, Jan 27, 2011 at 1:27 PM, Gudrun Lang <gu.lang <@t> gmx.at> wrote:

> Hi Akemi,
> we don't do Oil Red O, but the Sudan III in an aceton-ethanol mixture. To
> prevent the precipitates on the slide, we stain in a plastic coplin jar
> with
> a screwed tap. The dye-precipitates accumulate on the ground. We take care
> not to mix them up while putting in the slide. So we need no filtering
> before staining. We only stain frozens of lung without fixation.
>
> I don’t know the formula of Oil Red O solution, but perhaps it can be
> handled like the sudan III.
>
> bye
> Gudrun
>
> -----Ursprüngliche Nachricht-----
> Von: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Akemi
> Allison
> Gesendet: Mittwoch, 26. Jänner 2011 18:16
> An: histonet
> Betreff: [Histonet] Oil Red O for FS on Muscle
>
> Happy hump day!
>
> Does anyone have a good procedure for Oil Red O for FS on Muscle.
> The  procedure the lab I am working with is having a great deal of
> problems with their muscle biopsy panel.  I am trouble-shooting some
> of the issues for the 1-Step Trichrome 3.4 pH, NADH, AT Pase,  I am
> working with them with pH issues.
>
> The Oil Red O is very problematic.  They are mixing before use and
> filtering with 42 Watman paper, but there is a lot of residual
> background on the slides.  Also, they were not fixing the sections
> with 37% formaldehyde, even though the procedure calls for it.  Could
> you share who you get the stain from also.  Your assistance is
> greatly appreciated.
>
> Akemi
>
>
> Akemi Allison BS, HT (ASCP) HTL
> Director
> Phoenix Lab Consulting
> Tele: 408.335.9994
> E-Mail: akemiat3377 <@t> yahoo.com
>
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