[Histonet] RE: Rat Skin

[Fischer, Dolores]

The section of skin is too large. It should be no thicker than approximately 3mm. If you need to see the entire piece of skin, you should be able to submit multiple thin sections. A standard 12hr processing schedule works.  I am not familiar with zinc formalin, but the standard fixation time for formalin is, minimally 24hrs, therefore I would guess your fixation time is also too short.

Dolores    

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Shaw, Sharon
Sent: Thursday, January 27, 2011 10:39 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Rat Skin

Can anybody share their protocol on processing rat skin I seem to be having a problem with it being under processed. I fix the tissue for 11hrs in zinc formalin and then run a 12 hour processing schedule the skin size is 2.5cmx1cm.

Thanks,
Sharon

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