SPAM-LOW: [Histonet] in situ methodology part 2

Patsy Ruegg pruegg <@t>
Mon Jan 24 11:05:00 CST 2011


I have been testing a new ISH kit called RISH from BioCare.  It works on any
dig labeled mRna probe.  The method is designed for ffpe sections but I have
just adapted it to cryosections as well.  I can send you some pictures of
Tunnel and EBV on ffpe sections if you would like to see them.  Besides
Roche this is the only kit I know of out right now for ISH.  They sell the
kit with their probes, I think they only have EBV, Kappa and Lambda and
maybe CMV.  They say they are coming out with hpv probes this spring but I
have not seen them yet.  They will also sell the reagents without their
probe for researchers like you and me that use our own probes as long as
they are dig labeled.

Ventana has ISH for HPV but the probe is fitc labeled.  In research we
prefer dig labeled probes, at least my investigators do.



Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
wk email pruegg <@t>
web site

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-----Original Message-----
From: histonet-bounces <@t>
[mailto:histonet-bounces <@t>] On Behalf Of louise
Sent: Monday, January 24, 2011 12:31 AM
To: Histonet
Subject: SPAM-LOW: [Histonet] in situ methodology part 2

Hi all

just as clarification .....i need to brush up on my background of ISH - what
new products/methododolgies there might be on the market. The tissues I will
be using will either be rodent pups/embryos or formalin fixed/fresh adult

As for the probe - thats a stick point - not enough background information
to decide - but probably oligos.

So...the questions still stand:

   - where can i find good on-line material
   - Who offers the best application manuals - is Roche still int he market?

best regards

Louise Renton
Bone Research Unit
University of the Witwatersrand
South Africa
+27 11 717 2298 (tel & fax)
073 5574456 (emergencies only)
"There are nights when the wolves are silent and only the moon howls".
George Carlin
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