[Histonet] C3d
Pamela Marcum
mucram11 <@t> comcast.net
Thu Jan 20 12:42:16 CST 2011
Our patholgoists do not feel IHC gives them the discreet staining patterns they see with IF. They have tried IHC for C4d and did not like it. The only platform we have here is Ventana and that often slows us down on things as they may not have the antibody ready to use or a protocol for it.
Pam Marcum
----- Original Message -----
From: "Ronald Houston" <Ronald.Houston <@t> nationwidechildrens.org>
To: "Pamela Marcum" <mucram11 <@t> comcast.net>, "Richard Cartun" <Rcartun <@t> harthosp.org>
Cc: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, January 20, 2011 12:33:52 PM
Subject: RE: [Histonet] C3d
Pam,
We have been performing C4d by IHC for about 3 years now and C3d for many months on all our transplant biopsies. Obviously, being able to do this on paraffin obviates the need to have additional tissue for frozens (although I do understand the need for additional IF on kidney biopsies - but we're working on that!).
Currently we get both antibodies from Cell Marque and run them on our Bonds
Let me know if you need any more info.
Ronnie
Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Pamela Marcum
Sent: Thursday, January 20, 2011 1:28 PM
To: Richard Cartun
Cc: Histonet
Subject: Re: [Histonet] C3d
Hi Dr Cartun,
I hope anyone answering this will make it available for all of HistoNet. I would be very interested in the use of this antibody as we are already using C4d IF for kidney here.
Pamela Marcum
UAMS
Little Rock AR
----- Original Message -----
From: "Richard Cartun" <Rcartun <@t> harthosp.org>
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, January 20, 2011 11:26:29 AM
Subject: [Histonet] C3d
Is anyone using a "Commercially-available" C3d (not CD3) antibody for identifying antibody-mediated rejection in formalin-fixed cardiac transplant tissue? Thanks.
Richard
Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 545-1596 Office
(860) 545-2204 Fax
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
----- Original Message -----
From: "Richard Cartun" <Rcartun <@t> harthosp.org>
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, January 20, 2011 11:26:29 AM
Subject: [Histonet] C3d
Is anyone using a "Commercially-available" C3d (not CD3) antibody for identifying antibody-mediated rejection in formalin-fixed cardiac transplant tissue? Thanks.
Richard
Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 545-1596 Office
(860) 545-2204 Fax
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
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----- Original Message -----
From: "Ronald Houston" <Ronald.Houston <@t> nationwidechildrens.org>
To: "Pamela Marcum" <mucram11 <@t> comcast.net>, "Richard Cartun" <Rcartun <@t> harthosp.org>
Cc: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, January 20, 2011 12:33:52 PM
Subject: RE: [Histonet] C3d
Pam,
We have been performing C4d by IHC for about 3 years now and C3d for many months on all our transplant biopsies. Obviously, being able to do this on paraffin obviates the need to have additional tissue for frozens (although I do understand the need for additional IF on kidney biopsies - but we're working on that!).
Currently we get both antibodies from Cell Marque and run them on our Bonds
Let me know if you need any more info.
Ronnie
Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Pamela Marcum
Sent: Thursday, January 20, 2011 1:28 PM
To: Richard Cartun
Cc: Histonet
Subject: Re: [Histonet] C3d
Hi Dr Cartun,
I hope anyone answering this will make it available for all of HistoNet. I would be very interested in the use of this antibody as we are already using C4d IF for kidney here.
Pamela Marcum
UAMS
Little Rock AR
----- Original Message -----
From: "Richard Cartun" <Rcartun <@t> harthosp.org>
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, January 20, 2011 11:26:29 AM
Subject: [Histonet] C3d
Is anyone using a "Commercially-available" C3d (not CD3) antibody for identifying antibody-mediated rejection in formalin-fixed cardiac transplant tissue? Thanks.
Richard
Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 545-1596 Office
(860) 545-2204 Fax
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
----- Original Message -----
From: "Richard Cartun" <Rcartun <@t> harthosp.org>
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, January 20, 2011 11:26:29 AM
Subject: [Histonet] C3d
Is anyone using a "Commercially-available" C3d (not CD3) antibody for identifying antibody-mediated rejection in formalin-fixed cardiac transplant tissue? Thanks.
Richard
Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 545-1596 Office
(860) 545-2204 Fax
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
----------------------------------------- Confidentiality Notice:
The following mail message, including any attachments, is for the
sole use of the intended recipient(s) and may contain confidential
and privileged information. The recipient is responsible to
maintain the confidentiality of this information and to use the
information only for authorized purposes. If you are not the
intended recipient (or authorized to receive information for the
intended recipient), you are hereby notified that any review, use,
disclosure, distribution, copying, printing, or action taken in
reliance on the contents of this e-mail is strictly prohibited. If
you have received this communication in error, please notify us
immediately by reply e-mail and destroy all copies of the original
message. Thank you.
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