[Histonet] C3d

[Houston, Ronald]

Pam, 
We have been performing C4d by IHC for about 3 years now and C3d for many months on all our transplant biopsies. Obviously, being able to do this on paraffin obviates the need to have additional tissue for frozens (although I do understand the need for additional IF on kidney biopsies - but we're working on that!). 
Currently we get both antibodies from Cell Marque and run them on our Bonds
Let me know if you need any more info.
Ronnie

Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Pamela Marcum
Sent: Thursday, January 20, 2011 1:28 PM
To: Richard Cartun
Cc: Histonet
Subject: Re: [Histonet] C3d



Hi Dr Cartun, 



I hope anyone answering this will make it available for all of HistoNet.  I would be very interested in the use of this antibody as we are already using C4d IF for kidney here. 



Pamela Marcum 

UAMS 

Little Rock AR 


----- Original Message ----- 
From: "Richard Cartun" <Rcartun <@t> harthosp.org> 
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu> 
Sent: Thursday, January 20, 2011 11:26:29 AM 
Subject: [Histonet] C3d 

Is anyone using a "Commercially-available" C3d (not CD3) antibody for identifying antibody-mediated rejection in formalin-fixed cardiac transplant tissue?  Thanks. 

Richard 

Richard W. Cartun, MS, PhD 
Director, Histology & Immunopathology 
Director, Biospecimen Collection Programs 
Assistant Director, Anatomic Pathology 
Hartford Hospital 
80 Seymour Street 
Hartford, CT  06102 
(860) 545-1596 Office 
(860) 545-2204 Fax 



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----- Original Message ----- 
From: "Richard Cartun" <Rcartun <@t> harthosp.org> 
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu> 
Sent: Thursday, January 20, 2011 11:26:29 AM 
Subject: [Histonet] C3d 

Is anyone using a "Commercially-available" C3d (not CD3) antibody for identifying antibody-mediated rejection in formalin-fixed cardiac transplant tissue?  Thanks. 

Richard 

Richard W. Cartun, MS, PhD 
Director, Histology & Immunopathology 
Director, Biospecimen Collection Programs 
Assistant Director, Anatomic Pathology 
Hartford Hospital 
80 Seymour Street 
Hartford, CT  06102 
(860) 545-1596 Office 
(860) 545-2204 Fax 



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Histonet <@t> lists.utsouthwestern.edu 
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