[Histonet] Animal tissue processing
mab70 <@t> medschl.cam.ac.uk
Thu Jan 20 11:54:09 CST 2011
Generally rodent tissues require shorter times for processing. See the
"Animal Processing Manual" published by the NSH for a range of tried and
tested protocols. Below I have pasted a copy of my schedule which works
well for mouse tissues.
As you will see I designed it in the first instance for pancreas which
tends to harden on longer processes, now I use it for all my mouse
tissues, with the exception of intact heads (these require much longer,
i.e. 2 hours per station.) All my processes are developed using the
methods in the above mentioned manual as a guide.
My processor is a "dunk and dip" type of machine, the Leica TP1020.
I hope this helps.
PROGRAMME 4: FOR MOUSE PANCREAS
This programme can be run during the day as long as it is started early
enough; if not, a delay must be set in order that the samples are not
left in hot wax for extended times - refer to instrument manual.
Miss Margaret Blount
Metabolic Research Laboratories
Level 4 Institute of Metabolic Science
Box 289, Addenbrooke's Hospital
Hills Road, Cambridge, CB2 0QQ
Tel 01223 769061/336079
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gill,
Sent: 20 January 2011 16:49
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Animal tissue processing
I work in a hospital where we process human tissue. As a favor to a
friend the pathologist would like us to process animal tissue. My
questions are could we process the animal tissue on the same processor
with the human tissue? And Are there different processing times and
reagents for animal tissue? Thanks for any help you can give........
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