[Histonet] Animal tissue processing

[Margaret Blount]

Generally rodent tissues require shorter times for processing. See the
"Animal Processing Manual" published by the NSH for a range of tried and
tested protocols. Below I have pasted a copy of my schedule which works
well for mouse tissues.

 

As you will see I designed it in the first instance for pancreas which
tends to harden on longer processes, now I use it for all my mouse
tissues, with the exception of intact heads (these require much longer,
i.e. 2 hours per station.) All my processes are developed using the
methods in the above mentioned manual as a guide.

 

My processor is a "dunk and dip" type of machine, the Leica TP1020.

 

I hope this helps.

 

 

SOP 3

PROGRAMME 4: FOR MOUSE PANCREAS

COSHH CBH001

 

 


STATION


REAGENT


DURATION


VACUUM

1

Formalin/70% ethanol

30 mins

Y

2

80% Ethanol

30 mins

Y

3

90% Ethanol

20 mins

Y

4

Absolute ethanol/IMS

20 mins

Y

5

Absolute ethanol/IMS

30 mins

Y

6

Absolute ethanol/IMS

30 mins

Y

7

Histoclear II

20 mins

Y

8

Histoclear II

30 mins

Y

9

Histoclear II

30 mins

Y

10

Wax 

30 mins

Y

11

Wax

45 mins

Y

12

Wax

45 mins

Y

 

 

This programme can be run during the day as long as it is started early
enough; if not,  a delay must be set in order that the samples are not
left in hot wax for extended times - refer to instrument manual.

 

 

Good luck

 

Margaret

 

Miss Margaret Blount

Histology Manager

Metabolic Research Laboratories

Level 4 Institute of Metabolic Science

Box 289, Addenbrooke's Hospital

Hills Road, Cambridge, CB2 0QQ

 

Tel 01223 769061/336079

 

 

 

 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gill,
Caula A.
Sent: 20 January 2011 16:49
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Animal tissue processing

 

Hi All,

I work in a hospital where we process human tissue. As a favor to a

friend the pathologist would like us to process animal tissue. My

questions are could we process the animal tissue on the same processor

with the human tissue? And Are there different processing times and

reagents for animal tissue? Thanks for any help you can give........

 

Caula (HT)

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