[Histonet] RE: Caspase

[sgoebel <@t> mirnarx.com]

I cc'd (is that a word?) the histonet for you.  If you ever want to pose
a question to everyone email: histonet <@t> lists.utsouthwestern.edu.

PPE: Of course everyone has different levels of PPE to be worn, but I
think the standard rule is...
Gloves
Lab Coat
Mask (especially if cutting frozen lung tissue, it could have TB)
Eye Wear
You have to remember you need all of this because once the tissue hits
room temperature it is basically fresh unfixed tissue again.
I have cut frozen sections with no mask before and it was all ok, but I
always wore one with lung frozens.
Good Luck, hope this helped

Sarah Goebel, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912




-----Original Message-----
From: Setlak, Lisa [mailto:LSetlak <@t> childrensmemorial.org] 
Sent: Wednesday, January 19, 2011 10:08 AM
To: Sarah Goebel
Subject: RE: Caspase

Hi Sarah,
I don't have an answer to your question but was wondering if you could
post one for me- I've tried multiple times and it doesn't seem to go
through. Would you be able to tell me how to pose a question to the
whole group or could you possibly post one "What type of PPE is normally
worn when performing frozen sections"? Thanks in advance for your help.
Lisa Van Valkenberg
Histology Mgr. 
Children's Memorial Hospital
Chg,. IL

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
sgoebel <@t> mirnarx.com
Sent: Wednesday, January 19, 2011 9:53 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Caspase

Look...I am a plethora of questions today!!  Has anyone ever used the
antibody Caspase?  I hear lymph node is a good positive control, but
where have people purchased the antibody, and at what dilution for FFPE
tissue?  

Thanks

 

Sarah Goebel, BA, HT(ASCP)

Histotechnologist

Mirna Therapeutics

2150 Woodward Street

Suite 100

Austin, Texas  78744

(512)901-0900 ext. 6912

 

 

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