[Histonet] RE: Caspase

[John Shelley]

Hi Sarah,

I have used Cell Signaling antibody for Cleaved Caspase-3 (Asp175) Antibody #9661 is that what you are looking to do. I used it at a 1:100 dilution for 1 hr no heat with High pH. 

John S.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of sgoebel <@t> mirnarx.com
Sent: Wednesday, January 19, 2011 10:53 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Caspase

Look...I am a plethora of questions today!!  Has anyone ever used the
antibody Caspase?  I hear lymph node is a good positive control, but
where have people purchased the antibody, and at what dilution for FFPE
tissue?  

Thanks

 

Sarah Goebel, BA, HT(ASCP)

Histotechnologist

Mirna Therapeutics

2150 Woodward Street

Suite 100

Austin, Texas  78744

(512)901-0900 ext. 6912

 

 

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