[Histonet] Request for Coverslip Removal SOP

[histotech <@t> imagesbyhopper.com]

We put xylene in a jar, add the slide, cover the jar loosely and put it in a
warming oven.  Alternately, you could put the jar in a water bath.  The warm
xylene helps to soften the old mounting media.  This could take some time
though . old mounting media can be some pretty tough stuff!  ;o)

 

Good luck!

 

Michelle

 

 

From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Setlak, Lisa
Sent: Friday, January 14, 2011 3:15 PM
To: 'Sean McBride'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Request for Coverslip Removal SOP

 

Hi,
I don't have an actual SOP but we just soak in a jar of xylene until the
coverslip come off.
Lisa

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sean McBride
Sent: Friday, January 14, 2011 1:56 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Request for Coverslip Removal SOP
Importance: High

Hi colleagues,

I need to remove a glass coverslip from a mounted H&E biopsy slide with an
implant in order to run some biomaterial surface characterization studies.
Does anyone have a SOP that they would be willing to share for removing
glass coverslips without damaging the specimen?  Thanks in advance for all
of the great advice that I always get from the histonet.


Best regards,


~Sean McBride


Scientific Specialist
Bone Tissue Engineering Center
Carnegie Mellon Research Institute
Suite 4311
700 Technology Drive
Pittsburgh, PA 15219-3124

412-268-8275 (o)
412-915-1683 (m)
412-268-8275 (fax)
smcbride <@t> andrew.cmu.edu






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