[Histonet] decalcifying bone marrows after processing

Rene J Buesa rjbuesa <@t> yahoo.com
Fri Jan 14 15:42:21 CST 2011

If you what to do a histology work of quality, you cannot decalcify after processing, besides, what is the point?
It is preferable to use formic acid (even if it is worse than using EDTA) than having to struggle with a poor section produced and then trying to decalcify it.
This is typical of the ignorance of most pathologists about tissue processing things.
René J.

--- On Fri, 1/14/11, Powers, Kerry <PowersK <@t> ccmhonline.com> wrote:

From: Powers, Kerry <PowersK <@t> ccmhonline.com>
Subject: [Histonet] decalcifying bone marrows after processing
To: histonet <@t> lists.utsouthwestern.edu
Date: Friday, January 14, 2011, 4:31 PM

I was wondering if anyone has any experience with, or is it even possible to, decalcify bone marrows after they are processed.  Our pathologist would like to be able to process bone marrows the same day we receive them, but most of the time there just isn't enough time to allow for proper fixation and then proper decalcification.  She asked if we could process them and then decalcify and I have yet to find an answer to this question.  Please help!!

Thank you,

Kerry Powers
Comanche Country Memorial Hospital
Department of Pathology
3401 W Gore, Lawton OK 73505
(580) 355-8699 ext. 3359
Fax: (580) 585-5462
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