[Histonet] LR White embedding for Immuno EM

[Johnson, Teri]

Posted for a colleague of mine:

I am currently trying to get a GFP antibody to work on mouse intestinal crypts. I am using LR White embedded sections that were fixed in 4% PFA/0.1% GA in PBS. I put them in a flat embedding mold and cured in the oven at 60 C. Most of the LR White evaporated out overnight so I had to re-embed the sample. The paneth cells look as if they did not infiltrate well after re-embedding in gelatin capsules and I lost orientation.

Is there any way to recover this sample or will I need to start over again with new tissue sample?

Rhonda Trimble HT(ASCP)HTL, QIHC
Electron Microscopy Specialist
Stowers Institute for Medical Research
1000 E 50th Street
Kansas City, Missouri 64110
816-926-4346
rra <@t> stowers.org