[Histonet] Re: Histonet Digest, Vol 86, Issue 17

Mark Elliott Mark.Elliott <@t> hli.ubc.ca
Thu Jan 13 12:01:35 CST 2011

Thanks Mike
I have passed it on to the person who was asking.

Message: 1
Date: Wed, 12 Jan 2011 13:02:35 -0500
From: mtitford <@t> aol.com
Subject: [Histonet] Deparaffinization for EM
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <8CD809A06C3774E-13C4-538 <@t> webmail-d023.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"

Mark Elliot  asks about processing FFPE tissue for EM. Hayat published a method in his book we use. Briefly you dig out 1mm cube pieces of tissue from the block and rotate them in xylene for one hour, followed by absolute, 95%, 70%,50% alcohols for 15 minutes each and then into your EM buffer overnight, followed by your normal EM processing next day. The EM image looks pretty grotty but if your pathologist can make a diagnosis it is worth it.
(Hayat M.A. Principles and techniques of electron microscopy. 2ed edition. Baltimore. University Park Press. 1981 page 209)
In another method published years ago, someone by-passed the hydration steps by deparaffizing in osmium dissolved in xylene, then going straight to Epon.

Hope this helps

Michael Titford
Pathology USA
Mobile AL USA

This electronic message and any attachments are intended only for the use of the addressee and may contain information that is privileged and confidential.  Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system.

More information about the Histonet mailing list