[Histonet] Thyroid Smears

[DKBoyd <@t> chs.net]

Our procedure is as such:  The FNA of the thyroid is place in 5mls of 
plasmalyte ( an electrolyte solution).  We lyse the red blood cells and 
make cytospins. 

Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical 
Center I 
200 Medical Park Boulevard l Petersburg, Va.  23805 l T: 804-765-5050 l F: 
804-765-5582 l dkboyd <@t> chs.net







Fawn Bomar <Fawn.Bomar <@t> HalifaxRegional.com> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
01/12/2011 09:37 AM

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Subject
[Histonet] Thyroid Smears






Hello Everyone!

Happy New Years to all!  I have a question regarding the preparation of 
thyroid smears.  As of right now, we go up to the room and collect the 
thyroid sample.  The Pathologist makes the smears in the room and 
immediately puts them into 95% Isopropanol to fix.  We then complete the 
stain later on in the day.  The problem that we are encountering is that 
all of the blood and cells are coming off of the slides before we make it 
through the entire stain.  Does anyone have any suggestions or are willing 
to share the procedure that they use?  We had a couple of suggestions that 
we recommended to the Doctor but they were dismissed. I don't want to tell 
what are suggestions were so that the doctor cannot accuse us of 
influencing every one else's opinions.

Thank you in advance,
Fawn
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