[IHCRG] RE: [Histonet] batching controls

Tue Jan 11 14:07:17 CST 2011

No slides should be given to a pathologist until the positive controls have been reviewed and appropriately documented as "Satisfactory" by the Director of the laboratory or her/his designee.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax

>>> "Tim Higgins" <higginst <@t> amapath.com> 1/11/2011 1:09 PM >>>
What is the purpose of batching control?  Unless you are having a hard time
finding certain positive tissue types, it's good practice to run a positive
control with each case to ensure every slide shows the desired results.
Plus the patholigist have to call and verify that the control worked,
wasting their time. It doesn't waste money if you are attaching the patient
tissue to your control tissue, and the few that require a separate control
is just part of good patient care.

We have been around and around about this subject in places I have worked
and in the end batch controling is not the direction we want to go for
patient care.  If you are working in research enviroment maybe that is fine
but if it was my specimen, run a control.

Just my two cents!

Thanks,

Timothy Higgins, HT(ASCP) QIHC

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
ancillarypath <@t> mac.com 
Sent: Tuesday, January 11, 2011 11:57 AM
To: Sebree Linda A
Cc: histonet <@t> lists.utsouthwestern.edu; ihcrg <@t> googlegroups.com; Taylor,Jean
Subject: Re: [IHCRG] RE: [Histonet] batching controls

I agree with Linda. One positive control per *batch*, not per case. The
positive control tissue can be placed on one of the patient's specimens,
which saves you money on the detection system and antibody as well.

We should be careful also about treating different drawers with different
runs (such as the Bond, for example) as a separate batch, which requires its
own set of controls.

Some antibodies that have internal controls on every patient tissue do not
require external controls, such as vimentin, CD31, CD34, etc.

Predictive markers positive controls should always be the same controls, and
can only be changed after verifying that the new control is okay to run and
give you the desired signal.

Thanks,

Hadi Yaziji
================================
Hadi Yaziji, M.D., Medical Director
Vitro Molecular Laboratories
7480 SW 40 St, Suite 700  Miami, FL 33155 Tel 305-267-7979  F. 786-513-0175
www.vitromolecular.com 

On Jan 11, 2011, at 12:42 PM, Sebree Linda A wrote:

> Hi Jean,
> 
> We run one positive control for any given antibody that is ordered on 
> multiple cases and all are run at the same time on the same instrument.
> Whenever possible that control has the patient tissue of one of the 
> cases on it.  That case is referenced for the pathologists reading the 
> other cases with that antibody so they can find the positive control 
> if they feel the need.
> 
> 
> Linda A. Sebree
> University of Wisconsin Hospital & Clinics IHC/ISH Laboratory
> DB1-223 VAH
> 600 Highland Ave.
> Madison, WI 53792
> (608)265-6596
> 
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu 
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of 
> Taylor, Jean
> Sent: Tuesday, January 11, 2011 11:22 AM
> To: 'ihcrg <@t> googlegroups.com'; 'histonet <@t> lists.utsouthwestern.edu'
> Subject: [Histonet] batching controls
> 
> Hi Everyone,
> 
> I'd like to know how many labs batch their controls when the same 
> antibody is ordered on multiple cases. Do you run the control on a 
> separate slide, or with one of the cases ordered?
> 
> Thank you!
> 
> Jean Taylor, HT(ASCP)QIHC
> IHC Tech
> Meriter Health Services
> Madison, WI
> 
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