[IHCRG] RE: [Histonet] batching controls

ancillarypath <@t> mac.com ancillarypath <@t> mac.com
Tue Jan 11 11:57:15 CST 2011

I agree with Linda. One positive control per *batch*, not per case. The positive control tissue can be placed on one of the patient's specimens, which saves you money on the detection system and antibody as well.

We should be careful also about treating different drawers with different runs (such as the Bond, for example) as a separate batch, which requires its own set of controls.

Some antibodies that have internal controls on every patient tissue do not require external controls, such as vimentin, CD31, CD34, etc.

Predictive markers positive controls should always be the same controls, and can only be changed after verifying that the new control is okay to run and give you the desired signal.


Hadi Yaziji
Hadi Yaziji, M.D., Medical Director
Vitro Molecular Laboratories
7480 SW 40 St, Suite 700  Miami, FL 33155
Tel 305-267-7979  F. 786-513-0175

On Jan 11, 2011, at 12:42 PM, Sebree Linda A wrote:

> Hi Jean,
> We run one positive control for any given antibody that is ordered on
> multiple cases and all are run at the same time on the same instrument.
> Whenever possible that control has the patient tissue of one of the
> cases on it.  That case is referenced for the pathologists reading the
> other cases with that antibody so they can find the positive control if
> they feel the need. 
> Linda A. Sebree
> University of Wisconsin Hospital & Clinics
> IHC/ISH Laboratory
> DB1-223 VAH
> 600 Highland Ave.
> Madison, WI 53792
> (608)265-6596
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Taylor,
> Jean
> Sent: Tuesday, January 11, 2011 11:22 AM
> To: 'ihcrg <@t> googlegroups.com'; 'histonet <@t> lists.utsouthwestern.edu'
> Subject: [Histonet] batching controls
> Hi Everyone,
> I'd like to know how many labs batch their controls when the same
> antibody is ordered on multiple cases. Do you run the control on a
> separate slide, or with one of the cases ordered?
> Thank you!
> Jean Taylor, HT(ASCP)QIHC
> IHC Tech
> Meriter Health Services
> Madison, WI
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