[Histonet] custom-made polyclonal antibody doesn't stain
Barbara.Verstraeten <@t> ugent.be
Tue Jan 4 08:14:27 CST 2011
I had two antibodies made (in rabbit) for the same peptide.
It was the first time I did this : the firm designed the immunization
peptide, injected and bleed the rabbits and purified the antibody.
I have tested it on paraffin sections: all background, no staining where it
should be. I tried different retriever buffers and detection with dab and
I have tested it on western blot: a lot of background, dirty lanes. To get a
clearer view I performed a co-IP. Still not really great.
Can anybody help me on this issue?
I just want it to work for staining.
Thanks a lot in advance!
Barbara Verstraeten, Drs.
Evolutionary Developmental Biology
Department of Biology
K.L. Ledeganckstraat 35
tel: ++32/(0)9 264 52 31
fax: ++32/(0)9 264 53 44
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