[Histonet] IHC Equipment

[Victoria Baker]

In regards to the Ventana Ultra - if you are running PIN-4 or ISH
simultaneously on this instrument, the kits/ab's/probes  they require take
up a lot of space on the reagent carousel which limits the number of
antibodies you can put on and as runs can go some where around 6-hours you
could find yourself limited in what you can run without careful planning.

I'm a long time user and believer in Ventana, but BondMax and Intellipath do
have my loyalties as well.  It depends on the needs of your lab (volume/work
flow, available lab space and antibody library size), the staffing you have
available and their abilities with IHC/ISH.  I've done this before and the
only way to really know if an instrument will work in your lab is to
demo them and connect with other users of the instrument.

Vikki






On Fri, Feb 25, 2011 at 9:30 AM, Greg Dobbin <gvdobbin <@t> ihis.org> wrote:

> The Bond does do ISH. In fact it utilizes the same detection kit so you
> have only to buy the probes, not the additional detection kit that could
> (depending on your volume of ISH requests) expire.
> Greg
>
> Greg Dobbin, R.T.
> Chief Technologist, Anatomic Pathology
> Dept. of Laboratory Medicine,
> Queen Elizabeth Hospital,
> P.O. Box 6600
> Charlottetown, PE    C1A 8T5
> Phone: (902) 894-2337
> Fax: (902) 894-2385
>
> "I find that the harder I work, the
> more luck I seem to have."
> - Thomas Jefferson
>
>
> >>> "Sheila Fonner" <sfonner <@t> labpath.com> 2/25/2011 10:20 AM >>>
>  Cindy,
>
> We used to use the Dako stainer, and we still have it as a back-up if
> necessary, but we have recently (in the last year) bought a Ventana Ultra.
> You can put 30 slides at a time on it, but you do not have to batch the
> slides.  It is a continuous feed machine, which means that as soon as a
> slide is done, you can take it off and run something else.  You do not have
> to have all of the slides from a case together side by side.  It is
> bar-code
> driven and will find the slides no matter where you put them.  Each slide
> drawer runs independently of the others.  Ours has been wonderful.  The
> technical assistance is fantastic also.  They will help you to initially
> work up all of your antibodies.  You can use theirs, or you can use third
> party antibodies and place them in a prep-kit.  You can also use RTU or
> concentrates.  It's really up to you.  I would highly recommend it if you
> have a large volume.  We demo'd the Biocare Intellipath also.  I liked the
> machine, but it was really just a step up from the Dako.  Leica has the
> Bond
> instruments, which a lot of people like, but for us, it didn't work because
> we wanted to be able to do ISH.  Also, the Leica limits you to drawers of
> ten slides.  So when you load a drawer, with 1 slide or 10, you can't use
> it
> again until that run is finished.  Hope this helps.  If you have any
> questions, you can contact me.
> Have a great day!
>
> Sheila
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cynthia
> Pyse
> Sent: Wednesday, February 23, 2011 2:37 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] IHC Equipment
>
> Hi Histonetters
>
> I currently use a Dako stainer for my IHC staining. It is a work horse with
> very little problems. It is a older model that we may need to replace in
> the
> near future. What is everyone using out in histoland. I would be perfectly
> willing to purchase another Dako but I want to explore all avenues before
> making a decision. What are the pros and cons of the instruments any of you
> are using. How often is the machine down? What is the capacity? We run the
> Dako twice daily usually to the capacity of 48 slides. I would like to hear
> only from actual user of the instrumentation, no vendors please. This is
> only a fact finding e-mail. Thanks in advance for all your input.
>
> Cindy
>
>
>
> Cindy Pyse, CLT, HT (ASCP)
>
> Laboratory/Histology Supervisor
>
> X-Cell Laboratories
>
> 716-250-9235
>
> e-mail cpyse <@t> x-celllab.com
>
>
>
>
>
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