[Histonet] (no subject)

[Mark Turner]

Several of you have asked that I share our protocol for the P16 on the Ventana XT.  I apologize for the delay, but here is what we have worked up and it performs well in our lab:

CC1 Standard
Antibody incubation @ 37 degrees C. for 28 minutes
Amplification
Hematoxylin counterstain 4 minutes
Bluing Reagent 4 minutes

We place the commercial antibody from the CINtec kit in a prep kit and use it as an RTU.


Mark A. Turner, Ph.D. HT (ASCP) QIHC  |  Test Development Team Lead |  Department of Laboratory  Medicine and Pathology |  480-301-8026 |  Fax: 480-301-8372  | turner.mark <@t> mayo.edu    
Mayo Clinic in Arizona  |  13400 E. Shea Blvd.  |  Scottsdale, AZ 85259  |  www.mayoclinic.org