[Histonet] RE: Short term tissue storage PBS???
AGleiberman <@t> cbiolabs.com
Wed Feb 23 15:39:49 CST 2011
Nothing is changed, but storage in PBS does not affect neither morphology nor antigens. I used to keep some neutral formalin- or formaldehyde-PBS fixed specimens up to 1 year in sterile PBS at +4 C without any visible changes. So - don't worry. It will be fine.
Anatoli Gleiberman, PhD
Director of Histopathology
Cleveland Biolabs, Inc
73 High Street
Buffalo, NY 14203
e-mail: AGleiberman <@t> cbiolabs.com
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jo-Ann Bader, Ms.
Sent: Wednesday, February 23, 2011 3:57 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Short term tissue storage PBS???
It has come to my attention recently that some of our histology clients are submitting their samples in PBS after fixation, instead of 70% alcohol. When I ask them why they don't know and say they have been told to. I have always followed the procedure that after fixation specimens are stored, short term in 70% alcohol, until you are ready to process them. Has something changed lately that I am not aware of?
Goodman Cancer Centre
McGill University 1160 Pine Ave. W - Rm 312 (3355)
Montreal, QC, Canada
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