[Histonet] use of destained sections in IHC?

Sebree Linda A LSebree <@t> uwhealth.org
Wed Feb 23 13:59:38 CST 2011

We just take the coverslip/film off, hydrate to water and run for IHC
BUT many times the H&E slide is not a "+" slide so we end up losing
tissue.  It is not necessary to destain the H&E first.

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Robin
Sent: Wednesday, February 23, 2011 1:43 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] use of destained sections in IHC?

Hi all,


Is it possible to de-stain H&E stained  paraffin sections and re-use
for IHC staining??? Does anything special have to be done? Will it only
for some epitopes/stains? Does anyone have suggestions on how to do
this? I
am being asked to do this and don't want to waste my time if it won't
I would appreciate any suggestions anyone might have 


Thank you,




Robin R. Dean, Ph.D.

Senior Scientist & Study Director

Comparative Biosciences, Inc.

786 Lucerne Dr.

Sunnyvale, CA

(408) 738-8060

robin_dean <@t> compbio.com


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