[Histonet] use of destained sections in IHC?
Sebree Linda A
LSebree <@t> uwhealth.org
Wed Feb 23 13:59:38 CST 2011
We just take the coverslip/film off, hydrate to water and run for IHC
BUT many times the H&E slide is not a "+" slide so we end up losing
tissue. It is not necessary to destain the H&E first.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
600 Highland Ave.
Madison, WI 53792
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Robin
Sent: Wednesday, February 23, 2011 1:43 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] use of destained sections in IHC?
Is it possible to de-stain H&E stained paraffin sections and re-use
for IHC staining??? Does anything special have to be done? Will it only
for some epitopes/stains? Does anyone have suggestions on how to do
am being asked to do this and don't want to waste my time if it won't
I would appreciate any suggestions anyone might have
Robin R. Dean, Ph.D.
Senior Scientist & Study Director
Comparative Biosciences, Inc.
786 Lucerne Dr.
robin_dean <@t> compbio.com
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
More information about the Histonet